Journal: Journal of Pharmacy & Bioallied Sciences

Article Title: Cytotoxicity of Dhanwantharaarishtam on hiPSC Cells – An In vitro Study

doi: 10.4103/jpbs.jpbs_846_24

Figure Lengend Snippet: MTT assay of hiPSC cells treated with Dhanwantharaarishtam

Article Snippet: Human-induced pluripotent stem cells (hiPSCs) were only used for both cell viability and cytotoxic studies The hiPSC used in this study was obtained from National Centre for Cell Sciences (NCCS), Pune, India.

Techniques: MTT Assay

Journal: Journal of Pharmacy & Bioallied Sciences

Article Title: Cytotoxicity of Dhanwantharaarishtam on hiPSC Cells – An In vitro Study

doi: 10.4103/jpbs.jpbs_846_24

Figure Lengend Snippet: Cytotoxicity assay of hiPSC cells treated with Dhanwantharaarishtam

Article Snippet: Human-induced pluripotent stem cells (hiPSCs) were only used for both cell viability and cytotoxic studies The hiPSC used in this study was obtained from National Centre for Cell Sciences (NCCS), Pune, India.

Techniques: Cytotoxicity Assay

Journal: STAR Protocols

Article Title: Efficient generation of locus-specific human CAR-T cells with CRISPR/cCas12a

doi: 10.1016/j.xpro.2022.101321

Figure Lengend Snippet:

Article Snippet: DNA Clean & Concentrator-25 (Capped) , Zymo Research , Cat# D4033.

Techniques: Recombinant, Electroporation, Modification, SYBR Green Assay, Transfection, Enzyme-linked Immunosorbent Assay, Cytotoxicity Assay, DNA Extraction, Software, Real-time Polymerase Chain Reaction, Protein Purification, Plasmid Preparation, Synthesized, Flow Cytometry, Spectrophotometry

Journal: Cancer cell

Article Title: Direct Phosphorylation and Stabilization of MYC by Aurora B Kinase Promote T-cell Leukemogenesis

doi: 10.1016/j.ccell.2020.01.001

Figure Lengend Snippet: (A) Zebrafish embryos were injected respectively with the rag2:EGFP-Myc construct alone or in combination with rag2:AURKB. Representative images of GFP fluorescent microscopy analysis at 8 dpf are shown on the left. GFP fluorescence from the thymuses of EGFP-Myc (n = 13) and EGFP-Myc;AURKB fish (n = 14) were qualified and plotted on the right. Scale bar, 1 mm. Data are means ± SD, **p < 0.01, unpaired two-tailed Student’s t test.

Article Snippet: Zebrafish Studies Human AURKB coding sequences were ligated into the Rag2/pBKS plasmid ( Langenau et al., 2003 ) through BamHI and ClaI sites to generate the rag2:AURKB construct. rag2:EGFP-Myc alone ( Langenau et al., 2003 ), or in combination with rag2:AURKB , prepared in the microinjection solution [30 ng/μL DNA, 0.5×I-SceI buffer and 0.5 U/μL I-SceI meganuclease (NEB)] was injected into embryos of zebrafish at the one-cell stage of development.

Techniques: Injection, Construct, Microscopy, Fluorescence, Two Tailed Test

Journal: Cancer cell

Article Title: Direct Phosphorylation and Stabilization of MYC by Aurora B Kinase Promote T-cell Leukemogenesis

doi: 10.1016/j.ccell.2020.01.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Zebrafish Studies Human AURKB coding sequences were ligated into the Rag2/pBKS plasmid ( Langenau et al., 2003 ) through BamHI and ClaI sites to generate the rag2:AURKB construct. rag2:EGFP-Myc alone ( Langenau et al., 2003 ), or in combination with rag2:AURKB , prepared in the microinjection solution [30 ng/μL DNA, 0.5×I-SceI buffer and 0.5 U/μL I-SceI meganuclease (NEB)] was injected into embryos of zebrafish at the one-cell stage of development.

Techniques: Virus, Recombinant, CellTox Assay, Cytotoxicity Assay, Chromatin Immunoprecipitation, Plasmid Preparation, Luciferase, Reporter Assay, In Vitro, SYBR Green Assay, Software

Journal: Frontiers in Immunology

Article Title: A Novel Sample Selection Approach to Aid the Identification of Factors That Correlate With the Control of HIV-1 Infection

doi: 10.3389/fimmu.2021.634832

Figure Lengend Snippet: Descriptive statistics for all the proteins assessed.

Article Snippet: Fifty-two soluble analytes were quantified in plasma using a combination of six Meso Scale Discovery (MSD) human V-PLEX panels including the Angiogenesis Panel 1 (VEGF-A, VEGF-C, VEGF-D, Tie-2, Flt-1, PIGF, bFGF), TH17 Panel 1 (IL-17A, IL-21, IL-31, IL-27, IL-23, IL-22, MIP-3α), Chemokine Panel 1 (Eotaxin, MIP-1β, Eotaxin-3, TARC, IP-10, MIP-1α, IL-8, MCP-1, MDC, MCP-4), Cytokine Panel 1 (GM-CSF, IL-1α, IL-5, IL-7, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, VEGF-A), Cytokine Panel 2 (IL-1RA, IL-3, IL-9, IL-17A/F, IL-17B, IL-17C, IL-17D, TSLP), Proinflammatory Panel 1 (IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF-α), and Vascular Injury Panel 2 (SAA, CRP, VCAM-1, ICAM-1).

Techniques: